Low-Cost Methods for Molecular Characterization of Mutant Plants [electronic resource] : Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

• Tác giả: Mayada M Beshir, Bernhard J Hofinger, Owen A Huynh, Joanna Jankowicz-Cieslak, Robert G Laport, Bradley J Till
• Ngôn ngữ: eng
• ISBN: 9783319162584 (print)
• ISBN-13: 978-3319162591
• Ký hiệu phân loại: 572.82 Biochemistry
• Thông tin xuất bản: Cham : Springer International Publishing : Imprint: Springer, 2015
• Mô tả vật lý: X, 35 p. 9 illus., 3 illus. in color. , online resource.
• Bộ sưu tập: Tài liệu truy cập mở
• ID: 117352
• Chủ đề: 1. Cytology--Research_xMethodology; 2. Life sciences; 3. Nucleic acids; 4. Plant breeding

Tóm tắt

 This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues
  the extraction of high-quality DNA for downstream applications
  the extraction of single-strand-specific nucleases for single nucleotide polymorphism
  and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.