Chapter 48 In Vivo Bioluminescence Imaging to Assess Compound Efficacy Against Trypanosoma brucei

• Tác giả: Michael Barrett, Jeremy Mottram, Elmarie Myburgh, Ryan Ritchie
• Ngôn ngữ: eng
• ISBN-13: 978-1071602935
• ISBN: 9781071602942_48
• ISBN-13: 978-1071602966
• Ký hiệu phân loại: 616.9363 Other diseases
• Thông tin xuất bản: Springer Nature, 2020
• Mô tả vật lý: 1 electronic resource (17 p.)
• Bộ sưu tập: Tài liệu truy cập mở
• ID: 224179
• Chủ đề: 1. Biology, life sciences

Tóm tắt

Traditional animal models for human African trypanosomiasis rely on detecting Trypanosoma brucei brucei parasitemia in the blood. Testing the efficacy of new compounds in these models is cumbersome because it may take several months after treatment before surviving parasites become detectable in the blood. To expedite compound screening, we have used a Trypanosoma brucei brucei GVR35 strain expressing red-shifted firefly luciferase to monitor parasite distribution in infected mice through noninvasive wholebody bioluminescence imaging. This protocol describes the infection and in vivo bioluminescence imaging of mice to assess compound efficacy against T. brucei during the two characteristic stages of disease, the hemolymphatic phase (stage 1) and the encephalitic or central nervous system phase (stage 2).