A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 630.1 Agriculture and related technologies
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2021
• Mô tả vật lý: Size: p. 7637-7642 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 259738
• Chủ đề: 1. 60 applied life sciences; 2. Imaging; 3. Probes; 4. Stimulated raman scattering; 5. Sugars; 6. Transport

Tóm tắt

 Sucrose is the main saccharide used for longdistance transport in plants and plays an essential role in energy metabolism
  however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small ( 50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.