Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 546 Inorganic chemistry
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: Article No. 158102 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260344
• Chủ đề: 1. 36 materials science; 2. 43 particle accelerators; 3. 60 applied life sciences

Tóm tắt

We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x-rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.