In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 612.3 Digestion
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 596-603 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260380
• Chủ đề: 1. 60 applied life sciences; 2. Calcium phosphates; 3. Dental enamel; 4. Fluoride; 5. Tooth remineralization; 6. X-ray microtomography

Tóm tắt

 Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo)
  2% NaF neutral gel applied for 1 minute (Fluoride 1 min)
  CPP-ACP applied for 3 min (ACP 3 min)
  and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (g<
 sub>
 HAp<
 /sub>
 .cm<
 sup>
 -3<
 /sup>
 ), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<
 0.05). Fluoride gel presented higher %SHR followed by ACP 3 min (p<
 0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<
 0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<
 0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.