A synthetic biochemistry platform for cell free production of monoterpenes from glucose [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 547.6 Aromatic compounds
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: Article No. 15526 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260470
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Enzymes; 4. Metabolic engineering; 5. Metabolic pathways; 6. Synthetic biology

Tóm tắt

 Cell-free systems designed to perform complex chemical conversions of biomass to biofuels or commodity chemicals are emerging as promising alternatives to the metabolic engineering of living cells. We design a system comprises 27 enzymes for the conversion of glucose into monoterpenes that generates both NAD(P)H and ATP in a modified glucose breakdown module and utilizes both cofactors for building terpenes. Different monoterpenes are produced in our system by changing the terpene synthase enzyme. The system is stable for the production of limonene, pinene and sabinene, and can operate continuously for at least 5 days from a single addition of glucose. We also obtain conversion yields 495% and titres 415 g l<
 sup>
 -1<
 /sup>
 . The titres are an order of magnitude over cellular toxicity limits and thus difficult to achieve using cell-based systems. Overall, these results highlight the potential of synthetic biochemistry approaches for producing bio-based chemicals.