Imaging of Hydrogel Microsphere Structure and Foreign Body Response Based on Endogenous X-Ray Phase Contrast [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 578.7 Organisms characteristic of specific kinds of environments
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016
• Mô tả vật lý: Size: p. 1038-1048 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260713
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Hydrogels; 4. Microcomputed tomography; 5. X-ray phase contrast

Tóm tắt

Transplantation of functional islets encapsulated in stable biomaterials has the potential to cure Type I diabetes. However, the success of these materials requires the ability to understand their stability in vivo. Imaging techniques that enable monitoring of biomaterial performance are critical to further development in the field. In this study, we demonstrate for the first time that X-ray phase contrast (XPC) imaging techniques enable 3D imaging and evaluation of islet volume, alginate hydrogel structure and local soft tissue response. Islets were encapsulated in alginate systems prepared in methods used in clinical trials and implanted in a rodent omentum pouch model as a treatment for type I diabetes. Microbeads were imaged with XPC prior to implantation and following implantation into an omentum pouch. Islets could be identified within alginate beads and the islet volume quantified. Omental adipose tissue could be distinguished from inflammatory regions resulting from implanted beads. Individual beads and the local encapsulation response were visualized and quantifiable. Measurements were in agreement with histology. The 3D structure of the microbeads could be characterized with XPC and failed beads could also be identified. These results point to the substantial potential of XPC as a tool for imaging biomaterials in small animal models.