Nucleic acid encoding a self-assembling split-fluorescent protein system [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 572.8 Biochemical genetics
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Medium: ED : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261141
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences

Tóm tắt

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.