Toward production of jet fuel functionality in oilseeds [electronic resource] : Identification of FatB acyl-acyl carrier protein thioesterases and evaluation of combinatorial expression strategies in <i>Camelina</i> seeds

• Ngôn ngữ: eng
• Ký hiệu phân loại: 630.7 Education, research, related topics
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: p. 4251-4265 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261158
• Chủ đề: 1. 60 applied life sciences; 2. Camelina; 3. Cuphea; 4. Fatb acyl-acp thioesterase; 5. Jet fuel oilseed; 6. Medium-chain fatty acid

Tóm tắt

 Seeds of members of the genus <
 i>
 Cuphea<
 /i>
  accumulate medium-chain fatty acids (MCFAs
  8:0?14:0). MCFA- and palmitic acid- (16:0) rich vegetable oils have received attention for jet fuel production, given their similarity in chain length to Jet A fuel hydrocarbons. Studies were conducted to test genes, including those from <
 i>
 Cuphea<
 /i>
 , for their ability to confer jet fuel-type fatty acid accumulation in seed oil of the emerging biofuel crop <
 i>
 Camelina sativa<
 /i>
 . Transcriptomes from <
 i>
 Cuphea viscosissima<
 /i>
  and <
 i>
 Cuphea pulcherrima<
 /i>
  developing seeds that accumulate >
 90% of C8 and C10 fatty acids revealed three <
 i>
 FatB<
 /i>
  cDNAs (<
 i>
 CpuFatB3<
 /i>
 , <
 i>
 CvFatB1<
 /i>
 , and <
 i>
 CpuFatB4<
 /i>
 ) expressed predominantly in seeds and structurally divergent from typical FatB thioesterases that release 16:0 from acyl carrier protein (ACP). Expression of <
 i>
 CpuFatB3<
 /i>
  and <
 i>
 CvFatB1<
 /i>
  resulted in <
 i>
 Camelina<
 /i>
  oil with capric acid (10:0), and <
 i>
 CpuFatB4<
 /i>
  expression conferred myristic acid (14:0) production and increased 16:0. Co-expression of combinations of previously characterized <
 i>
 Cuphea<
 /i>
  and California bay FatBs produced <
 i>
 Camelina<
 /i>
  oils with mixtures of C8?C16 fatty acids, but amounts of each fatty acid were less than obtained by expression of individual <
 i>
 FatB<
 /i>
  cDNAs. Increases in lauric acid (12:0) and 14:0, but not 10:0, in <
 i>
 Camelina<
 /i>
  oil and at the <
 i>
 sn-2<
 /i>
  position of triacylglycerols resulted from inclusion of a coconut lysophosphatidic acid acyltransferase specialized for MCFAs. RNA interference (RNAi) suppression of <
 i>
 Camelina<
 /i>
  ?-ketoacyl-ACP synthase II, however, reduced 12:0 in seeds expressing a 12:0-ACP-specific FatB. Here, <
 i>
 Camelina<
 /i>
  lines presented here provide platforms for additional metabolic engineering targeting fatty acid synthase and specialized acyltransferases for achieving oils with high levels of jet fuel-type fatty acids.