Physical and biological regulation of neuron regenerative growth and�network formation on recombinant dragline silks [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 612.6 Reproduction, development, maturation
• Thông tin xuất bản: Bethesda, Md. : Oak Ridge, Tenn. : National Institutes of Health (U.S.) ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: p. 137-146 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261224
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Biomaterial; 4. Neural cell; 5. Recombinant protein; 6. Silk; 7. Tissue engineering

Tóm tắt

In this paper, recombinant spider silks produced in transgenic goat milk were studied as cell culture matrices for neuronal growth. Major ampullate spidroin 1 (MaSp1) supported neuronal growth, axon extension and network connectivity, with cell morphology comparable to the gold standard poly-lysine. In addition, neurons growing on MaSp1 films had increased neural cell adhesion molecule (NCAM) expression at both mRNA and protein levels. The results indicate that MaSp1 films present useful surface charge and substrate stiffness to support the growth of primary rat cortical neurons. Moreover, a putative neuron-specific surface binding sequence GRGGL within MaSp1 may contribute to the biological regulation of neuron growth. These findings indicate that MaSp1 could regulate neuron growth through its physical and biological features. Finally, this dual regulation mode of MaSp1 could provide an alternative strategy for generating functional silk materials for neural tissue engineering.