Reconstructing each cell's genome within complex microbial communities�??dream or reality? [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 579.2 *Viruses and subviral organisms
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2015
• Mô tả vật lý: Size: Article No. 771 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 261251
• Chủ đề: 1. 60 applied life sciences; 2. Environmental microbiology; 3. Genome completeness; 4. Genome quality; 5. Microbial ecology; 6. Microbiology; 7. Multiple displacement amplification; 8. Single-cell sequencing

Tóm tắt

As the vast majority of microorganisms have yet to be cultivated in a laboratory setting, access to their genetic makeup has largely been limited to cultivation-independent methods. These methods, namely metagenomics and more recently single-cell genomics, have become cornerstones for microbial ecology and environmental microbiology. One ultimate goal is the recovery of genome sequences from each cell within an environment to move toward a better understanding of community metabolic potential and to provide substrate for experimental work. As single-cell sequencing has the ability to decipher all sequence information contained in an individual cell, this method holds great promise in tackling such challenge. Methodological limitations and inherent biases however do exist, which will be discussed here based on environmental and benchmark data, to assess how far we are from reaching this goal.