Effects of sample treatments on genome recovery via single-cell genomics [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 577.8 Synecology and population biology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Size: p. 2546-2549 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262140
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Fish; 4. Sample preservation; 5. Single-cell genomics

Tóm tắt

 It is known that single-cell genomics is a powerful tool for accessing genetic information from uncultivated microorganisms. Methods of handling samples before single-cell genomic amplification may affect the quality of the genomes obtained. Using three bacterial strains we demonstrate that, compared to cryopreservation, lower-quality single-cell genomes are recovered when the sample is preserved in ethanol or if the sample undergoes fluorescence <
 i>
 in situ<
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  hybridization, while sample preservation in paraformaldehyde renders it completely unsuitable for sequencing.