PanDaTox [electronic resource] : A tool for accelerated metabolic engineering

• Ngôn ngữ: eng
• Ký hiệu phân loại: 660.6 Biotechnology
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2012
• Mô tả vật lý: Size: p. 218-221 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262245
• Chủ đề: 1. 60 applied life sciences; 2. Metabolic engineering, toxic genes, pandatox, gene cloning, synthetic biology

Tóm tắt

Metabolic engineering is often facilitated by cloning of genes encoding enzymes from various heterologous organisms into E. coli. Such engineering efforts are frequently hampered by foreign genes that are toxic to the E. coli host. We have developed PanDaTox (www.weizmann.ac.il/pandatox), a web-based resource that provides experimental toxicity information for more than 1.5 million genes from hundreds of different microbial genomes. The toxicity predictions, which were extensively experimentally verified, are based on serial cloning of genes into E. coli as part of the Sanger whole genome shotgun sequencing process. PanDaTox can accelerate metabolic engineering projects by allowing researchers to exclude toxic genes from the engineering plan and verify the clonability of selected genes before the actual metabolic engineering experiments are conducted.