Determining mutant spectra of three RNA viral samples using ultra-deep sequencing [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 572.8 Biochemical genetics
• Thông tin xuất bản: Livermore, Calif : Oak Ridge, Tenn. : Lawrence Livermore National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2012
• Mô tả vật lý: Size: PDF-file: 13 pages
• Bộ sưu tập: Metadata
• ID: 262253
• Chủ đề: 1. 59 basic biological sciences; 2. 60 applied life sciences; 3. Animals; 4. Mutants; 5. Mutations; 6. Rna; 7. Spectra; 8. Viruses

Tóm tắt

RNA viruses have extremely high mutation rates that enable the virus to adapt to new host environments and even jump from one species to another. As part of a viral transmission study, three viral samples collected from naturally infected animals were sequenced using Illumina paired-end technology at ultra-deep coverage. In order to determine the mutant spectra within the viral quasispecies, it is critical to understand the sequencing error rates and control for false positive calls of viral variants (point mutantations). I will estimate the sequencing error rate from two control sequences and characterize the mutant spectra in the natural samples with this error rate.