Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids [electronic resource]

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Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 662.6 Fuels

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2022

Mô tả vật lý: Size: Article No. 3870 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 262456

Economically viable production of cellulosic biofuels requires operation at high solids loadings?on the order of 15 wt%. To this end we characterize Nature?s ability to deconstruct and utilize mid-season switchgrass at increasing solid loadings using an anaerobic methanogenic microbiome. This community exhibits undiminished fractional carbohydrate solubilization at loadings ranging from 30 g/L to 150 g/L. Metaproteomic interrogation reveals marked increases in the abundance of specific carbohydrate-active enzyme classes. Significant enrichment of auxiliary activity family 6 enzymes at higher solids suggests a role for Fenton chemistry. Stress-response proteins accompanying these reactions are similarly upregulated at higher solids, as are ?-glucosidases, xylosidases, carbohydrate-debranching, and pectin-acting enzymes?all of which indicate that�removal of deconstruction inhibitors is important for observed undiminished solubilization. Our work provides insights into the mechanisms by which natural microbiomes effectively deconstruct and utilize lignocellulose at high solids loadings, informing the future development of defined cultures for efficient bioconversion.
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