Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in <em>Rhodosporidium toruloides</em> [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 664.7 Grains, other seeds, their derived products
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2019
• Mô tả vật lý: Size: p. 2103-2109 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262832
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. Crisprcas9; 4. Genome editing; 5. Metabolic engineering

Tóm tắt

 The oleaginous yeast <
 em>
 Rhodosporidium toruloides<
 /em>
  is considered a promising candidate for production of chemicals and biofuels thanks to its ability to grow on lignocellulosic biomass, and its high production of lipids and carotenoids. However, efforts to engineer this organism are hindered by a lack of suitable genetic tools. In this paper, we report the development of a CRISPR/Cas9 system for genome editing in <
 em>
 R. toruloides<
 /em>
  based on a fusion 5S rRNA?tRNA promoter for guide RNA (gRNA) expression, capable of greater than 95% gene knockout for various genetic targets. Additionally, multiplexed double-gene knockout mutants were obtained using this method with an efficiency of 78%. Finally, this tool can be used to accelerate future metabolic engineering work in this yeast.