Deciphering mixotrophic <em>Clostridium formicoaceticum</em> metabolism and energy conservation [electronic resource] : Genomic analysis and experimental studies

• Ngôn ngữ: eng
• Ký hiệu phân loại: 621.48 Nuclear engineering
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2018
• Mô tả vật lý: Size: p. 1687-1694 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 262891
• Chủ đề: 1. 09 biomass fuels; 2. Acetate; 3. Acetogen; 4. Clostridium formicoaceticum; 5. Genomic analysis

Tóm tắt

 <
 em>
 Clostridium<
 /em>
  formicoaceticum, a Gram-negative mixotrophic homoacetogen, produces acetic acid as the sole metabolic product from various carbon sources, including fructose, glycerol, formate, and CO<
 sub>
 2<
 /sub>
 . Its genome of 4.59-Mbp contains a highly conserved Wood-Ljungdahl pathway gene cluster with the same layout as that in other mixotrophic acetogens, including <
 em>
 Clostridium aceticum<
 /em>
 , <
 em>
 Clostridium carboxidivorans<
 /em>
 , and <
 em>
 Clostridium ljungdahlii<
 /em>
 . For energy conservation, <
 em>
 C. formicoaceticum<
 /em>
  does not have all the genes required for the synthesis of cytochrome or quinone used for generating proton gradient in H<
 sup>
 +<
 /sup>
 -dependent acetogens such as <
 em>
 Moorella thermoacetica<
 /em>
 
  instead, it has the Rnf system and a Na<
 sup>
 +<
 /sup>
 -translocating ATPase similar to the one in <
 em>
 Acetobacterium<
 /em>
  woodii. Furthermore its growth in both heterotrophic and autotrophic media were dependent on the sodium concentration. <
 em>
 C. formicoaceticum<
 /em>
  has genes encoding acetaldehyde dehydrogenases, alcohol dehydrogenases, and aldehyde oxidoreductases, which could convert acetyl-CoA and acetate to ethanol and butyrate to butanol under excessive reducing equivalent conditions.