From model to crop [electronic resource] : functional characterization of <em>SPL8</em> in <em>M.�truncatula</em> led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả:

Ngôn ngữ: eng

Ký hiệu phân loại: 630.1 Agriculture and related technologies

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017

Mô tả vật lý: Size: p. 951-962 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263139

 Biomass yield, salt tolerance and drought tolerance are important targets for alfalfa (<
 em>
 Medicago sativa L.<
 /em>
 ) improvement. <
 em>
 Medicago truncatula<
 /em>
  has been developed into a model plant for alfalfa and other legumes. By screening a Tnt1 retrotransposon-tagged <
 em>
 M. truncatula<
 /em>
  mutant population, we identified three mutants with enhanced branching. Branch development determines shoot architecture which affects important plant functions such as light acquisition, resource use and ultimately impacts biomass production. Molecular analyses revealed that the mutations were caused by Tnt1 insertions in the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 8 (SPL8) gene. The <
 em>
 M. truncatula<
 /em>
  spl8 mutants had increased biomass yield, while overexpression of SPL8 in <
 em>
 M. truncatula<
 /em>
  suppressed branching and reduced biomass yield. Scanning electron microscopy (SEM) analysis showed that SPL8 inhibited branching by directly suppressing axillary bud formation. Based on the <
 em>
 M. truncatula<
 /em>
  SPL8 sequence, alfalfa SPL8 (MsSPL8) was cloned and transgenic alfalfa plants were produced. MsSPL8 down-regulated or up-regulated alfalfa plants exhibited similar phenotypes to the <
 em>
 M. truncatula<
 /em>
  mutants or overexpression lines, respectively. Specifically, the MsSPL8 down-regulated alfalfa plants showed up to 43% increase in biomass yield in the first harvest. The impact was even more prominent in the second harvest, with up to 86% increase in biomass production compared to the control. Furthermore, downregulation of MsSPL8 led to enhanced salt and drought tolerance in transgenic alfalfa. Results from this research offer a valuable approach to simultaneously improve biomass production and abiotic stress tolerance in legumes.
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 71010608 | Email: tt.thuvien@hutech.edu.vn

Copyright @2020 THƯ VIỆN HUTECH