Pre-Steady-State Kinetics of Catalytic Intermediates of an [FeFe]-Hydrogenase [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 668.3 Adhesives and related products
• Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: p. 2145-2150 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 263356
• Chủ đề: 1. 08 hydrogen; 2. 09 biomass fuels; 3. 59 basic biological sciences; 4. Biofuels (including algae and biomass); 5. Solar (fuels)

Tóm tắt

 [FeFe]-hydrogenases catalyze the reversible production of hydrogen gas from protons and electrons, but the mechanism of catalysis is still the subject of debate. In this report, we describe a pre-steady-state photoreduction methodology for correlating putative intermediates to the reactivity of an [FeFe]-hydrogenase from <
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 Thermotoga maritima<
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 . In this method, MV+ is rapidly formed photochemically by pulsed laser excitation and the intermolecular ET and active site dynamics are followed by nanosecond time-resolved visible and infrared spectroscopy, respectively. The results kinetically validate the H<
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 , H<
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 red<
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 , and H<
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  intermediates, strongly supporting a vast body of literature on their involvement in proton reduction.