Ferredoxin [electronic resource] : NAD+ oxidoreductase of Thermoanaerobacterium saccharolyticum and its role in ethanol formation [Identification of a ferredoxin NAD+ oxidoreductase of Thermoanaerobacterium saccharolyticum and its role in ethanol formation]

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Ngôn ngữ: eng

Ký hiệu phân loại: 668.5 Perfumes and cosmetics

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: p. 7134-7141 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263392

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Ferredoxin:NAD
+
oxidoreductase (NADH-FNOR) catalyzes the transfer of electrons from reduced ferredoxin to NAD
+
. This enzyme has been hypothesized to be the main enzyme responsible for ferredoxin oxidization in the NADH-based ethanol pathway in 
Thermoanaerobacterium saccharolyticum

however, the corresponding gene has not yet been identified. Here, we identified the Tsac_1705 protein as a candidate FNOR based on the homology of its functional domains. We then confirmed its activity 
in vitro
with a ferredoxin-based FNOR assay. To determine its role in metabolism, the 
tsac_1705
gene was deleted in different strains of T. saccharolyticum. In wild-type 
T. saccharolyticum
, deletion of 
tsac_1705
resulted in a 75% loss of NADH-FNOR activity, which indicated that Tsac_1705 is the main NADH-FNOR in 
T. saccharolyticum
. When both NADH- and NADPH-linked FNOR genes were deleted, the ethanol titer decreased and the ratio of ethanol to acetate approached unity, indicative of the absence of FNOR activity. As a result, we tested the effect of heterologous expression of Tsac_1705 in Clostridium thermocellum and found improvements in both the titer and the yield of ethanol.

1. 09 biomass fuels
2. 59 basic biological sciences
3. Ethanol production
4. Ferredoxins
5. Metabolic engineering

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Ferredoxin [electronic resource] : NAD<sup>+</sup> oxidoreductase of <i>Thermoanaerobacterium saccharolyticum</i> and its role in ethanol formation [Identification of a ferredoxin NAD<sup>+</sup> oxidoreductase of <i>Thermoanaerobacterium saccharolyticum</i> and its role in ethanol formation]