Development of a Rhodobacter capsulatus self-reporting model system for optimizing light-dependent, [FeFe]-hydrogenase-driven H2 production [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 630.1 Agriculture and related technologies

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Science ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: p. 291-297 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263423

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The photosynthetic bacterium 
Rhodobacter capsulatus
normally photoproduces H
2
as a by-product of its nitrogenase-catalyzed nitrogen-fixing activity. Such H
2
production, however, is expensive from a metabolic perspective, requiring nearly four times as many photons as the equivalent algal hydrogenase-based system. Here we report the insertion of a Clostridium acetobutylicum [FeFe]-hydrogenase and its three attendant hydrogenase assembly proteins into an 
R. capsulatus
strain lacking its native uptake hydrogenase. Further, this strain is modified to fluoresce upon sensing H
2
. The resulting strain photoproduces H
2
and self-reports its own H
2
production through fluorescence. Furthermore, this model system represents a unique method of developing hydrogenase-based H
2
production in 
R. capsulatus
, may serve as a powerful system for in vivo directed evolution of hydrogenases and hydrogenase-associated genes, and provides a means of screening for increased metabolic production of H
2
.

1. 09 biomass fuels
2. High-throughput screening
3. Nitrogenase
4. Photobiohydrogen
5. R. capsulatus h2 sensor

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Development of a <i>Rhodobacter capsulatus</i> self-reporting model system for optimizing light-dependent, [FeFe]-hydrogenase-driven H<sub>2</sub> production [electronic resource]