Use of De Novo transcriptome libraries to characterize a novel oleaginous marine Chlorella species during the accumulation of triacylglycerols [electronic resource]

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Ngôn ngữ: eng

Ký hiệu phân loại: 621.48 Nuclear engineering

Thông tin xuất bản: Washington, D.C. : Oak Ridge, Tenn. : United States. Dept. of Energy. Office of Energy Efficiency and Renewable Energy ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2016

Mô tả vật lý: Size: Article No. e0147527 : , digital, PDF file.

Bộ sưu tập: Metadata

ID: 263520

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Here, marine chlorophytes of the genus 
Chlorella
are unicellular algae capable of accumulating a high proportion of cellular lipids that can be used for biodiesel production. In this study, we examined the broad physiological capabilities of a subtropical strain (C596) of 
Chlorella
sp. ?SAG-211-18? including its heterotrophic growth and tolerance to low salt.We found that the alga replicates more slowly at diluted salt concentrations and can grow on a wide range of carbon substrates in the dark.We then sequenced the RNA of 
Chlorella
strain C596 to elucidate key metabolic genes and investigate the transcriptomic response of the organism when transitioning from a nutrient-replete to a nutrient-deficient condition when neutral lipids accumulate. Specific transcripts encoding for enzymes involved in both starch and lipid biosynthesis, among others, were up-regulated as the cultures transitioned into a lipid-accumulating state whereas photosynthesis-related genes were down-regulated. Transcripts encoding for two of the up-regulated enzymes?a galactoglycerolipid lipase and a diacylglyceride acyltransferase?were also monitored by reverse transcription quantitative polymerase chain reaction assays. The results of these assays confirmed the transcriptome-sequencing data. The present transcriptomic study will assist in the greater understanding, more effective application, and efficient design of 
Chlorella
-based biofuel production systems.

1. 09 biomass fuels
2. 59 basic biological sciences
3. Lipases
4. Lipids
5. Transcriptome analysis

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Use of <i>De Novo</i> transcriptome libraries to characterize a novel oleaginous marine <i>Chlorella</i> species during the accumulation of triacylglycerols [electronic resource]