The proposed project built on the foundation of over several years years of intensive and ground-breaking R&D work at Cellana's Kona Demonstration Facility (KDF). Phycological and engineering solutions were provided to tackle key cultivation issues and technical barriers limiting algal biomass productivity identified through work conducted outdoors at industrial (1 acre) scale. The objectives of this project were to significantly improve algal biomass productivity and reduce operational cost in a seawater-based system, using results obtained from two top-performing algal strains as the baseline while technically advancing and more importantly, integrating the various unit operations involved in algal biomass production, processing, and refining.