Improvement of enzymatic saccharification of Populus and switchgrass by combined pretreatment with steam and wet�disk�milling [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 668.4 Plastics
• Thông tin xuất bản: Oak Ridge, Tenn. : Oak Ridge, Tenn. : Oak Ridge National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2014
• Mô tả vật lý: Size: p. 782-789 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 264858
• Chủ đề: 1. 09 biomass fuels; 2. 59 basic biological sciences; 3. Hot-compressed water; 4. Populus; 5. Wet disk milling

Tóm tắt

 In this study, to reduce the recalcitrance of lignocellulosic biomass for subsequent biological processing, we pretreated energy crop feedstocks with mild steam treatment (ST
  130 and 150 �C for 60 min) and wet disk milling (WDM). We tested two phylogenetically different, but typical energy crop feedstocks: <
 i>
 Populus trichocarpa<
 /i>
  and switchgrass (<
 i>
 Panicum virgatum<
 /i>
 ). WDM after ST facilitated the fibrillation of both types of biomass, resulting in an increase of specific surface area, improved enzymatic saccharification yield, and decrease in cellulose crystallinity. Lastly, after steam treatment at 150 �C followed by 17 cycles of WDM, enzymatic hydrolysis resulted in almost complete glucan to glucose conversion in both feedstocks.