Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo [electronic resource]

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Tác giả: Thomas Harry Sharp

Ngôn ngữ:

ISBN-13: 978-3319021591

Ký hiệu phân loại: 543 Analytical chemistry

Thông tin xuất bản: Cham : Springer International Publishing : Imprint: Springer, 2014.

Mô tả vật lý: XVIII, 150 p. 85 illus., 55 illus. in color. , online resource.

Bộ sưu tập: Tài liệu truy cập mở

ID: 309561

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his PhD, Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
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