Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P [electronic resource] : Implications for FMD Vaccine Development

• Ngôn ngữ: eng
• Ký hiệu phân loại: 636.3907 Smaller ruminants Sheep
• Thông tin xuất bản: Oak Ridge, Tenn. : Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2017
• Mô tả vật lý: Size: Article No. e00924-17 : , digital, PDF file.
• Bộ sưu tập: Metadata
• ID: 260401
• Chủ đề: 1. 3c protease; 2. 59 basic biological sciences; 3. 60 applied life sciences; 4. Adenoviruses; 5. Escherichia coli; 6. Et al; 7. Foot-and-mouth disease virus; 8. Gaussia luciferase; 9. Stopgo translation; 10. Virology; 11. Vlp

Tóm tắt

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 ABSTRACT<
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 The foot-and-mouth disease virus (FMDV) afflicts livestock in more than 80 countries, limiting food production and global trade. Production of foot-and-mouth disease (FMD) vaccines requires cytosolic expression of the FMDV 3C protease to cleave the P1 polyprotein into mature capsid proteins, but the FMDV 3C protease is toxic to host cells. To identify less-toxic isoforms of the FMDV 3C protease, we screened 3C mutants for increased transgene output in comparison to wild-type 3C using a<
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 Gaussia<
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 luciferase reporter system. The novel point mutation 3C(L127P) increased yields of recombinant FMDV subunit proteins in mammalian and bacterial cells expressing P1-3C transgenes and retained the ability to process P1 polyproteins from multiple FMDV serotypes. The 3C(L127P) mutant produced crystalline arrays of FMDV-like particles in mammalian and bacterial cells, potentially providing a practical method of rapid, inexpensive FMD vaccine production in bacteria.<
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 IMPORTANCE<
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 The mutant FMDV 3C protease L127P significantly increased yields of recombinant FMDV subunit antigens and produced virus-like particles in mammalian and bacterial cells. The L127P mutation represents a novel advancement for economical FMD vaccine production.<
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