Report for the NGFA-5 project. [electronic resource]

• Ngôn ngữ: eng
• Ký hiệu phân loại: 363 Other social problems and services
• Thông tin xuất bản: Livermore, Calif : Oak Ridge, Tenn. : Lawrence Livermore National Laboratory ; Distributed by the Office of Scientific and Technical Information, U.S. Dept. of Energy, 2011
• Mô tả vật lý: Size: PDF-file: 108 pages
• Bộ sưu tập: Metadata
• ID: 262309
• Chủ đề: 1. 54 environmental sciences; 2. 59 basic biological sciences; 3. 60 applied life sciences; 4. Aerosols; 5. Bacillus; 6. Bacterial spores; 7. Biological warfare agents; 8. Comparative evaluations; 9. Dna sequencing; 10. Et al; 11. Evaluation

Tóm tắt

The objective of this project is to provide DHS a comprehensive evaluation of the current genomic technologies including genotyping, TaqMan PCR, multiple locus variable tandem repeat analysis (MLVA), microarray and high-throughput DNA sequencing in the analysis of biothreat agents from complex environmental samples. To effectively compare the sensitivity and specificity of the different genomic technologies, we used SNP TaqMan PCR, MLVA, microarray and high-throughput illumine and 454 sequencing to test various strains from B. anthracis, B. thuringiensis, BioWatch aerosol filter extracts or soil samples that were spiked with B. anthracis, and samples that were previously collected during DHS and EPA environmental release exercises that were known to contain B. thuringiensis spores. The results of all the samples against the various assays are discussed in this report.